Date: Thu, 25 Aug 2005  16:08:10  \-0500
To: flybase-updates@XXXX
From: Kevin Cook <kcook@XXXX>
Subject: Isolation and characterization of Df(3L)BSC123
Isolation and characterization of Df(3L)BSC123
Rachel Andrade and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC123 was isolated as a FLP recombinase-induced recombination
event involving P{XP}d03930 and PBac{WH}f05230. The deletion was
isolated as a chromosome lacking miniwhite markers in progeny of
P{hsFLP}1, w1118; P{XP}d03930/PBac{WH}f05230 females crossed to
w1118; P{w+mC=hs-hid}2, wgSp-1/CyO males. These females were
heat shocked as larvae as described in Parks et al., Nature Genetics
36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding
and succeeding generations maintained the original genetic background
of the Exelixis insertion stocks (Thibault et al, Nature Genetics 36:
283-287, 2004; FBrf0175002). The recombination event generated the
genetic element P+PBac{XP5.WH5}BSC123 from the segment of P{XP}d03930
to the left of its FRT site and the segment of PBac{WH}f05230 to the
right of its FRT site. Its presence was verified using the PCR
methods and primers described in Parks et al. Exelixis, Inc.
determined the insertion site of P{XP}d03930 to be at Release 3
genomic coordinate 1775286 on chromosome arm 3L and the insertion
site of PBac{WH}f05230 to be at Release 3 genomic coordinate 1786783
on arm 3L. The Gene Disruption Project determined the insertion site
of P{XP}d03930 to be at Release 3 genomic coordinate 1775476 on arm
3L and the insertion site of PBac{WH}f05230 to be at Release 3
genomic coordinate 1786781 on arm 3L. The cytological breakpoints of
Df(3L)BSC123 predicted from these coordinates are 62B4;62B4. It
failed to complement Cdc37e4D and Df(3L)Exel6088.
__________________________________________________________
Kevin Cook, Ph.D. Bloomington Drosophila Stock Center
Department of Biology http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University 812-856-1213
1001 E. Third St. 812-855-2577 (fax)
Bloomington, IN 47405-7005 kcook@XXXX