Date: Fri, 29 Jul 2005  15:34:27  \-0700
Subject: bursicon mutant mistake
From: Susan McNabb <smcnabb@XXXX>
To: Beverley Matthews <bmatthew@XXXX>, Hans-Willi Honegger
<h.willi.honegger@XXXX>
Dear Beverley,
I reassessed our data on bursZ1140 in response to your query (below) to
Dr. Honegger, as my group was responsible for characterization of the burs
mutants. I found that both the location of the mutation and the nature of
the amino acid change in the paper are incorrect. The correct location is at
residue 63 (not 97). The mutation changes a tyrosine to a cysteine (not
threonine to cysteine). The mutation is a single base change in which the
TAT codon is mutated to TGT.
To clarify, the sequence around the mutated site is VLQYPGC. The Y is
mutated to C.
I apologize for this error. Thanks for bringing this to our attention.
Yours truly,
Susan McNabb
\--
Susan L. McNabb, Research Asst Prof
Department of Biology, Box 351800
University of Washington
Seattle, WA 98195-1800
tel: (206)543-6513
fax: (206)616-2011
email: smcnabb@XXXX
Dear Dr. Honegger,
I am a curator for FlyBase and am currently capturing the genomic sites
of the burs mutations that you reported in your 2004 Current Biology
paper, to appear in the genome maps in FlyBase. I have a question about
the bursZ1140 allele. The text says that each of the five reported
mutations has a single base change in CG13419, but bursZ1140 is
reported as a change from Thr to Cys, which would require conversion of
two of the bases of the ACC (Thr) codon to get a TGC (Cys) codon. Can
you please let me know what the actual base changes that you observed
are?
Thank you for your help,
Sincerely,
Beverley Matthews
Curator, FlyBase-Harvard
bmatthew@XXXX