Date: Tue, 21 Sep 2004  10:26:23  \-0500
To: rd120@XXXX
From: Kevin Cook <kcook@XXXX>
Subject: P{Ubi-Sara} construct and insertion
The following information accompanied stocks donated to the Bloomington
Stock Center by Davie Van Vactor and Karl Johnson, Harvard Medical School
(8/04).
The P{Ubi-Sara} construct and insertion described in Johnson et al., 2004
(Curr. Biol.  14:499-504 ; FBrf0174485) were obtained from the laboratory of
Michael O'Connor. A 4 kb Sara cDNA fragment from clone LD13137 was blunt
end ligated into the StuI site of pP{Ubi-CaSpeR} (FBmc0002737), a vector
described in Brummel et al., 1994 (Cell  78:251-261 ; FBrf0072708).
P{Ubi-Sara.J}2 is a second chromosome insertion.
P{UAS-Sdc.J}3 is a homozygous viable and fertile, third chromosome insertion.
__________________________________________________________
Kevin Cook, Ph.D. Bloomington Drosophila Stock Center
Department of Biology http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University 812-856-1213
1001 E. Third St. 812-855-2577 (fax)
Bloomington, IN 47405-3700 kcook@XXXX