Date: Fri, 4 Feb 2005  19:36:21  \-0500 (EST)
From: Kathy Matthews <matthewk@XXXX>
Subject: progenitor of P{CARY.attP}
To: flybase-updates@XXXX
P{CARY.attP} is almost certainly derived from P{Car20y} and therefore
carries y+t7.7. In trying to determine the genotype details for the
Bloomington stock records, I asked Matt Fish if this was the case and
he provided this quote from Amy Groth:
'The plasmid pCaryP was created by removing attP from pTAattP (GROTH
et al. 2000) by EcoRI digestion, filling in with T4 DNA polymerase and
cloning it into the SmaI site of pCary, a plasmid containing the yellow
body color gene (gift of the Bruce Baker lab).'
P{Car20y} is the obvious suspect for pCary, and it is the only transgene
construct in FlyBase that is consistent with 'Car' in the plasmid symbol
and the characterisitcs of pCary.