Date: Thu, 08 Jul 2004  12:06:00  \-0500
To: flybase-updates@XXXX
From: Kevin Cook <kcook@XXXX>
Subject: Chang constructs and insertions
The following information accompanied stocks donated to the Bloomington
Stock Center by Henry Chang, Yale University (6/04).
In P{UAS-Rab4-mRFP}, the Rab4 (=CG4921) coding sequence is fused to the
mRFP sequence. The fusion protein labels early endosomes.
P{UAS-Rab4-mRFP}2 is a homozygous viable and fertile, second chromosome
insertion.
In P{UAS-Rab11-GFP}, the Rab11 (=CG5771) coding sequence is fused to the
EGFP coding sequence. The fusion protein labels recycling endosomes.
P{UAS-Rab11-GFP}2 is a homozygous viable and fertile, second chromosome
insertion.
In P{UAS-Grasp65-GFP}, the CG7809 coding sequence is fused to the EGFP
coding sequence. The fusion protein labels Golgi complex.
P{UAS-Grasp65-GFP}2 is a homozygous viable and fertile, second chromosome
insertion.
P{UAS-Grasp65-GFP}3 is a third chromosome insertion.
__________________________________________________________
Kevin Cook, Ph.D. Bloomington Drosophila Stock Center
Department of Biology http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University 812-856-1213
1001 E. Third St. 812-855-2577 (fax)
Bloomington, IN 47405-3700 kcook@XXXX