Date: Tue, 30 Mar 2004  13:09:42  \-0600
From: Hugh Robertson <hughrobe@XXXX>
To: 'Gillian Millburn (Genetics)' <gm119@XXXX>
Subject: Re: missing e-mail
Thanks for remembering this, Gillian, here's the basic story. The three
exon gene is below with introns in lower case.
ATGGAACAGGCGTACAGTACAGCTTCCCGACTGCGTGGGATTTGCGTTTGTGGATTGGCCATTTCGGTGTATTCACTGTA
TGTGAAAATGAAACTAAAGGAGGATGAGAACTATAGGCCAATGTGCGACGTTAACGATAATATAAGCTGCTCGCTGGTTT
TTAAATCGGGgtaggtaagattataatatatacaatataaatttaatgaaataattttccatcagCTATGGTGATGGCTT
TGGTCTGGGTAACATAACCCAAGTAAATGCTCCCAACGGAGCCATCGGCTGTGCATTTTATATCCTGTACTTCTTGAGCT
gtacgtaaatttcttagtacaaactaaactaaatttaataagcatttgttttcagCTTTCTTTAATCATCGTTGGCTGTG
CCTGGTCCAATTGATAGTATGCACTTTGACATTACTTCTCTGCGTTTATCTGGGTTTCCTGCTGATTCTCGTGTTTTACG
ATTTCTGTTTGGTATGCGTGACCATCTATTTCATACACACATGGCTCTTTCAGGAAGTCCTAAGGCGATATAGACGCCTT
TATATGTAA
The encoded protein is
MEQAYSTASRLRGICVCGLAISVYSLYVKMKLKEDENYRPMCDVNDNISCSLVFKSGYGDGFGLGNITQVNAPNGAIGCA
FYILYFLSSFFNHRWLCLVQLIVCTLTLLLCVYLGFLLILVFYDFCLVCVTIYFIHTWLFQEVLRRYRRLYMZ
This is the Drosophila homolog of the newly described human and other
mammalian VKORC1 gene encoding vitamin K epoxide reductase. There are
nice pseudoobscura and Anopheles orthologs, and the latter has ESTs.
It is currently partly annotated by Hild et al in the TPA as HDC06808,
however they splice from the middle of the third exon to a fourth
distant downstream exon, which is not right and might even be a gene
fusion. This downstream gene does have ESTs supporting it, so you might
want to look into it too.
There are no ESTs or any other data I can find for this gene in Drosophila.
Thanks,
Hugh