From rd120@XXXX Mon Jun 09  11:51:00  2003
To: xavier.morinXXXX, william.chiaXXXX
Subject: Helping FlyBase: PTT lines
Dear Xavier and Bill,
I'm writing about your PTT paper
\*x FBrf0141725 == Morin et al., 2001, Proc. Natl. Acad. Sci. USA 98(26):
15050--15055
This paper will give rise to three transposon records, for P{PTT-GA},
P{PTT-GB}, P{PTT-GC} in FlyBase. There will also be insertion records
for P{PTT-GA}III-1b, P{PTT-GB}III-3a etc from Table 1.
The thing is that I would also like to make records for the insertion
lines in Tables 2 and 3, however to do this I need to know which of
P{PTT-GA}, P{PTT-GB} or P{PTT-GC} is inserted in each case. If I knew
this I would make an allele of the gene in question, e.g. for G5, if it
was an insertion of P{PTT-GA}, I would make an allele of Tm2, called
Tm2G5, with an associated insertion P{PTT-GA}Tm2G5. For those
lines not associated with a known or predicted gene, the insertion
would go into FlyBase as a simple insertion, e.g. P{PTT-GA}G50.
Would you be able to tell me which transposon is inserted in each
line? We may as well get these lines explicitly instantiated in FlyBase
now, or else we'll just have to do them piecemeal later on. I've
included a list of the lines at the bottom, so that it is easy for you
to put the info in a reply message.
Looking forward to hearing from you.
With best wishes,
Rachel.
\----------------------------------------------------------------------
Rachel Drysdale, Ph.D.
FlyBase (Cambridge),
Department of Genetics,
University of Cambridge,
Downing Street, email: rd120@XXXX
Cambridge, CB2 3EH, Ph : 01223-333963
UK. FAX: 01223-333992
FlyBase: http://fly.ebi.ac. uk:7081 /
\----------------------------------------------------------------------
From morin@XXXX Thu Jun 19  17:24:39  2003
To: rd120@XXXX
Subject: protein trap
Dear Rachel,
I attach a table which summarises the info you need. Unfortunately, since
at some point we pooled some cages, some lines arised from a mixture of
GA/B/C progenitors: they are labelled with a '?'. three lines have both a
PTT id and a question mark: these came from pools, but I have had other
subsequent inserts in the same loci and introns, with clearly identified
GA-B-C PTT progenitors, so I assume they were derived from the same PTT.
regards,
Xavier MORIN, PhD
Developmental Biology Institute of Marseilles (IBDM)
Case 907, Campus de Luminy
13288 Marseille Cedex 9
FRANCE
tel: 33 (0)4 91 26 97 46
fax: 33 (0)4 91 26 97 48
morin@XXXX
www.ibdm.univ-mrs.fr
\-----------------------
Known genes:
G5 C
G7 A
G29 ?
G44 ?
G53 A(?)
G74 A(?)
G109 C
G126 B
G129 A
G138 A
G158 B
G169 ?
G259 A
G262 B
G280 C
G305 A
G409 B
G430 A
G454 ?
\-----------------------
Predicted genes:
G9 A
G38 ?
G88 A
G89 A
G93 B
G112 C
G119 A
G147 B
G180 C
G189 B 
G196 B
G198 A
G245 ?
G264 C
G271 A
G282 C
G365 C
\-----------------------
No known/predicted gene:
G50 ?
G108 C
G116 A
G123 ?
G154 B
G157 A
G231 C
G258 A
G270 A
G318 B
G357bis ?
G145 B
G215 B
G214 C
G260 B
G276 B
G281 B
G284 B
G287 B
G304 B
G341 ?
G357 B
G360 ?
G361 B
G370 ?
G377 B
G392 C
G413 A
G419 C
G428 C (?)
\-----------------------