Date: Mon, 24 Feb 2003  14:46:31  \-0800
To: Chihiro Yamada <cy200@XXXX>
From: Ranjiv Khush <khush@XXXX>
Subject: Re: FlyBase Query (cy1790)
Hi Chihiro,
You've picked out some mistakes in the paper that I've corrected
below. If you can note these corrections as a personal communication
to flybase, I would appreciate it.
Thanks,
Ranjiv
>Dear Dr Khush,
>
>I am currently curating your paper for FlyBase:
>
>Khush et al., 2002, Curr. Biol. 12(20): 1728--1737
>
>I have a few questions I was hoping you could answer for me.
>
>dCullin1
>========
>In your materials and methods you describe an allele,
>dCullin1l(2)02074. This confuses me for a number of reasons.
>
>The only gene we have in our records that has been called Cullin1 or
>dCul1 is a gene which is called lin19 in FlyBase, aka CG1877 or
>l(2)k01207. This has been placed at 43F. This gene was called dCul1
>in Bocca et al., 2001, Biochem. biophys. Res. Commun. 286(2): 357--364
>
>We currently have a gene called l(2)02074, which has an allele
>l(2)0207402074, caused by an insertion, P{PZ}l(2)0207402074. This
>has been placed at 39EF by insitu. I BLASTed the flanking sequence for
>this insertion, and again it is placed at 39-40.
>
>Which annotation/gene do you mean when you use the symbol dCullin1 in
>your Curr. Biol. paper?
>
>Do you mean the gene at 39EF which is affected by the insertion
>P{PZ}l(2)0207402074, or do you mean CG1877 at 43F?
>
>Do you have data on the P{PZ}l(2)0207402074 insertion that FlyBase
>does not know about? Any more information you have about this would be
>greatly appreciated.
I made a mistake: l(2)02074 is an insertion in cul-2 or CG1512. The
mutation that I described in the paper is l(2)k01207, the insertion
in dCullin1.
..
Ranjiv Khush
Department of Microbiology and Immunology
Stanford University School of Medicine
Stanford, CA 94305-5124 USA
phone: (650) 724-8064
FAX: (650) 725-6757
email: khush@XXXX