From: R.D.Saunders@XXXX
To: gm119@XXXX
Cc: lesley.mclellan@XXXX
Subject: RE: FlyBase query: Glutamate-Cysteine ligase
Date: Thu, 13 Jun 2002  15:10:49  \+0100
Hi Gillian,
Happy to answer your query. Some enzyme commission renamed the enzyme
subunits, so that:
gamma Gltuamylcysteine synthetase, or gammaGCS became glutamate cysteine
ligase, or GCL
The two subunits formerly had the suffixes h for 'heavy' and l for 'light'.
These now become GCLC (for GCL catalytic subunit), and GCLM (for GCL
modifier subunit).
So GCSh = GCLC and GCSl = GCLM. We prefixed these with Dm to indicate
Drosophila melanogaster.
Hope this clarifies the matter! ..
Robert
Dr Robert D C Saunders
Lecturer in Molecular Biology
Department of Biological Sciences
The Open University
Walton Hall
Milton Keynes MK7 6AA
Tel: 01908-654069
http://www.open.ac.uk/science/biosci/research/molecular-genetics/molgen.html
> \-----Original Message-----
> From: Gillian Millburn <up> mailto:gm119 @XXXX</up>
> Sent: 13 June 2002  15:03 
> To: r.d.saunders@XXXX
> Cc: gm119@XXXX
> Subject: FlyBase query: Glutamate-Cysteine ligase
>
> Dear Dr. Saunders,
>
> I am curating your paper for FlyBase:
>
> Fraser et al., 2002, J. Biol. Chem. 277(2): 1158--1165
>
> and I have a couple of questions.
>
> A. is the 'DmGCLM' gene in this paper, the same gene as that called
> 'DmGCSl' in your abstract:
>
> Saunders et al., 2001, Europ. Dros. Res. Conf. 17: F34
>
> 'Glutathione synthesis in Drosophila: molecular and genetic
> characterisation of gamma-glutamylcysteine synthetase.'
>
> I am pretty sure it is the same gene but I just wanted to check.
>
> B. I'd like to sort out the nomenclature of the
> 'gamma-glutamylcysteine
> synthetase' genes in FlyBase.
>
> At the moment we have:
>
> 1. for the catalytic subunit:
>
> gene symbol: Gcsh
> full name: gamma-Glutamylcysteine synthetase catalytic subunit
>
> this corresponds to CG2259 and is the gene that you called 'DmGCS' in
> your paper Saunders and McLellan, 2000, FEBS Lett. 467(2-3): 337--340
> and 'DmGCSh' in your abstract Saunders et al., 2001, Europ. Dros. Res.
> Conf. 17: F34.
>
> In your 2002 JBC paper you call this 'DmGCLC' for
> 'glutamate-cysteine ligase catalytic subunit'
>
> 2. for the regulatory subunit (assuming that I'm right in A. above and
> 'DmGCLM' in your JBC paper is the same gene as 'DmGCSl' in your EDRC
> abstract):
>
> gene symbol: Gcsl
> full name: gamma-Glutamylcysteine synthetase regulatory subunit
>
> this corresponds to EST GH01757, which means that it is predicted gene
> CG4919.
>
> In your 2002 JBC paper you call this 'DmGCLM' for
> 'glutamate-cysteine ligase modifier subunit'
>
> are these 2 symbols above the ones that you want to be the valid
> symbols in FlyBase, or would you rather have the valid symbols be the
> ones you use in the 2002 JBC paper, i.e.
>
> 1. for the catalytic subunit:
>
> symbol: Gclc (or Gcl-c)
> full name: Glutamate-cysteine ligase catalytic subunit
>
> 2. for the regulatory subunit
>
> symbol: Gclm (or Gcl-m)
> full name: Glutamate-cysteine ligase modifier subunit
>
> Since your group is the first to have published on these two genes
> you can choose the final valid symbols for these genes in FlyBase.
> Could you let me know what you would like the symbols to be (they
> should be the symbols you intend to use in future publications),
>
> I look forward to hearing from you,
>
> Gillian
>
> \--------------------------------------------------------------
> Gillian Millburn.
>
> FlyBase (Cambridge),
> Department of Genetics,
> University of Cambridge,
> Downing Street, email: gm119@XXXX
> Cambridge, CB2 3EH, Ph : 01223-333963
> UK. FAX: 01223-333992
> \--------------------------------------------------------------
>