From delidaki@XXXX Tue Mar 12  12:42:10  2002
To: flybase-updates@XXXX
Subject: mapping of Gal4 line
Hello
we were interested to map the Gal4-C253 line, so my student (Nikolaos
Giagtzoglou) did plasmid rescue and sequencing. The sequence we
obtained is below. By BLAST we found that the transposon has inserted
153bp upstream of the scabrous gene. This agrees very well with the
proneural cluster expression pattern that this driver gives. I'm
sending this information to include in FLyBase, as it will probably be
of use to the Drosophila community.
Regards
Christos
CCCCCGCCCGGGGATCAGATCCGCGGCCGGCCGCATAGGCCACTAGTGGATCTGGATCCTCTAG
CTAG|AGCTTTGCGTACTCGCAAATTATTAAAAATAAAACTTTAAAAATAATTTCGTCTAATTAA
TATTATGAGTTAATTCAAACCCCACGGACATGCTAAGGGTTAATCAACAATCATATCGCTGTCT
CACTCAGACTCAATACGACACTCAGAATACTATTCCTTTCACTCGCACTTATTGCAAGCATACG
TTAAGTGGATGTCTCTTGCCGACGGGACCACCTTATGTTATTTCATCATGA|GCGGGGGAATTGC
CGAGGAAGAGTGCCAAAGAGAGCAGCCACCTGCCCGAAGCATGCAACAAGCAGGGAAGAGAGCC
ACAAAGAGAACGCATCAAGTTAAGAGAAAATCAATGAATAAACTCGATGTTAGATGCCCGAAAA
ATGTCCTTTGAAATGTTTGCTATTACCAAAGATCATGTCTTTAAGGCAGCAGTTACCATTCGGA
GTTTCAGATAGCGAACGAGAGCAACACAAAATGGCGGTCTAGTTACCCGGTGCTTCTTTTTTGC
ACTCATCCTGCTCCTGACACATGGGCGCGGTTGCGGTGAGACTACAACGAAAACAAACTGCAAC
GAAAACGGCAGATGTTCGGGCCCATAGGCAGGCAGGCAGACAGAAGGCGAAGTGCGAAAACATG
CTGATGTGCTAGATATACTGCAAGACGAACAAAAACCAGACGG|GGCCCAAATGGTCCCAGTCCG
GTAGTTGCAAGG
To first | : plasmid DNA
Between |1 and |2: P element
Between |2 and |3: genomic DNA
After |3: bad quality sequence