From kcook@XXXX Thu Oct 19  16:29:46  2000
To: flybase-updates@XXXX
Subject: P{UAS-proPO} insertions
From: Kevin Cook, Bloomington Stock Center
Subject: P{UAS-proPO} insertions
John True, Artyom Kopp and Sean Carroll will describe the construction of
P{UAS-proPO} in detail in an upcoming publication. The following
information accompanied P{UAS-proPO} stocks they donated to the Stock
Center (9/00). This UAS construct in pUAST contains the cDNA for
prophenoloxidase (proPO). Expression of proPO allows the production of
melanin in the presence of dopamine and isopropanol. UAS-proPO is an
alternative reporter construct to UAS-lacZ or UAS-GFP. There are three
homozygous viable and fertile insertions, one for each major chromosomes:
P{w+mC=UAS-proPO}X
P{w+mC=UAS-proPO}2
P{w+mC=UAS-proPO}3
The insertions have not been mapped within each chromosome.
__________________________________________________________
Kevin Cook, Ph.D. Bloomington Drosophila Stock Center
Department of Biology http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University 812-855-5782
1001 E. Third St. 812-855-2577 (fax)
Bloomington, IN 47405-3700 kcook@XXXX