From rd120@XXXX Wed Dec 08  14:26:19  1999
To: gboul@XXXX
Subject: Helping FlyBase: P4 element
Dear Gabrielle,
I am curating your J. Neurosci. paper
Guo et al., 1999 (J. Neurosci. 19(19): 8435--8442)
and have a question for you. You mention (Fig 1) a P-element insertion
that you call P4. Could you tell me a little more about this insertion
please, such as what kind of element is inserted, and does it cause a
mutant phenotype due to the disruption of the L15 locus? Is it one of
the BDGP insertions? If so it will have an insertion identifier which
would be very useful in my curation so please could you let me know
what it is.
Many thanks for your help,
with best wishes,
Rachel.
From gboul@XXXX Wed Dec 08  19:33:37  1999
To: Rachel Drysdale (Genetics) <rd120@XXXX>
Subject: Re: Helping FlyBase: P4 element
Dear Rachel,
The P4 line was originally obtained from Peter Deak who is now in
Cambridge, not BDGP. His original number for this line is 750/2 and it is a
placw insertion. The original line is male and female sterile but it is
not clear if the sterility is associated with the P element insertion. The
Pelement itself is not in L15 and does not appear to disrupt the gene
although, as aforementioned, it remains possible that the sterility could
be associated with the Pelement insertion and affect this gene.
Best wishes,
Gabrielle
Gabrielle L. Boulianne, Ph.D.
Senior Scientist
Program in Developmental Biology
Hospital for Sick Children &
Associate Professor
Dept. Medical Genetics
University of Toronto
555 University Avenue
Toronto, Ontario
Canada M5G 1X8
Ph: 416-813-8701
Fax: 416-813-5086
email: gboul@XXXX