Personal communication from: Ulrich Schaefer, Herbert Jackle, Yuchun He, Hugo Bellen, Todd Laverty and Gerry Rubin
To: Bloomington Drosophila Stock Center
Subject: Goettingen lethals - set 3
Dated: 12 August 1999
Background: The lethal and semi-lethal alleles and P{lacW} insertions reported here were generated in the laboratory of
Herbert Jackle (Goettingen) in a screen for lethal P{lacW} hops into the X chromosome. In situ hybridizations to map
the P{lacW} insertions were carried out in the laboratory of Hugo Bellen (Houston). Hybridized chromosomes were read
by both the Bellen lab and by Todd Laverty (Rubin lab, Berkeley). The assumption that lethality in single insertion lines
is caused by the insertion has not been verified. lacZ expression patterns for these lines will be available from FlyView.
This communication includes revised insertion sites for some of the lines included in FBrf0108529, followed by new
alleles and transposon insertions.
Alleles with updated information (presumed to be caused by P{lacW} insertions):
Original Update
l(1)G0329[G0329] 1B7-10 ? -- multi-insert line, 1B7-10 and 9D
l(1)G0044[G0044] 2B1-4 2A3-B2
l(1)G0012[G0012] 2A1-2 2B1-4
l(1)G0014[G0014] 2C1-2 2C1-8
l(1)G0025[G0025] 5D1-2 5C5-8
l(1)G0233[G0233] 7D3-5 7D5-9
l(1)G0203[G0203] 7E5-6 7E5-10
l(1)G0137[G0137] 8F1-2, 9A1-3 8F4-9A1 (formerly multi-insert line)
l(1)G0126[G0126] 9F1-2 9E4-8
l(1)G0076[G0076] 9E7-8 9E5-8
l(1)G0065[G0065] 10B1-2 10A10-12
l(1)G0060[G0060] 11B1-4 11B14-C2
l(1)G0070[G0070] 17C1-2 17D
l(1)G0147[G0147] 3A3, 18C1-2 18B (formerly multi-insert line)
l(1)G0120[G0120] 18F1-2 18F
l(1)G0062[G0062] 19A1-2 18F1-2
Updated transposon insertions (from multi-insert lines):
Original Update
P{lacW}G0097a 4E 4DE
P{lacW}G0097b 9C1-2 9C
P{lacW}G0097c 12F1-3 12F
P{lacW}G0329a 1B7-10 (new)
P{lacW}G0329b 9D (new)
P{lacW}G0137a 8F1-2 eliminate
P{lacW}G0137b 9A1-3 eliminate
P{lacW}G0147a 3A3 eliminate
P{lacW}G0147b 8C1-2 eliminate
New Alleles (presumed to be caused by P{lacW} insertions):
l(1)G0378[G0378] ? -- multi-insert line: 1B7-10, 4E1-2
l(1)G0389[G0389] 1C
l(1)G0431[G0431] 2A
l(1)G0042[G0042] 2B1-8
l(1)G0401[G0401] 2B1-8
l(1)G0406[G0406] 2B1-8
l(1)G0158[G0158] 2D1-2
l(1)G0385[G0385] semi-lethal 2E
l(1)G0181[G0181] 3A1-4
l(1)G0374[G0374] 3A1-4
l(1)G0377[G0377] 3A1-4
l(1)G0445[G0445] ? -- multi-insert line: 3A1-4, 4C15-D2
l(1)G0152[G0152] ? -- multi-insert line: 3A1-4, 7E9-11
l(1)G0400[G0400] ? -- multi-insert line: 3A1-4, 8F
l(1)G0380[G0380] ? -- multi-insert line: 3A1-4, 9E3-8
l(1)G0175[G0175] ? -- multi-insert line: 3A1-4, 10F
l(1)G0434[G0434] ? -- multi-insert line: 3A1-4, 17C5-7
l(1)G0412[G0412] 3A3-4
l(1)G0375[G0375] semi-lethal ? -- multi-insert line: 3A3-4, 14B3-4
l(1)G0151[G0151] 3A3-6
l(1)G0335[G0335] 3B1-2
l(1)G0139[G0139] semi-lethal 3D1-4
l(1)G0354[G0354] 3D3-4
l(1)G0343[G0343] 3D5-E2
l(1)G0373[G0373] 3E1-4
l(1)G0167[G0167] semi-lethal 3E6-F2
l(1)G0299[G0299] semi-lethal 3F1-2
l(1)G0433[G0433] 3F1-2
l(1)G0336[G0336] 4B
l(1)G0387[G0387] 4C5-8
l(1)G0273[G0273] 4C7-8
l(1)G0338[G0338] 4C15-D2
l(1)G0432[G0432] semi-lethal 4C15-D3
l(1)G0248[G0248] semi-lethal 4D1-2
l(1)G0334[G0334] 4D1-2
l(1)G0371[G0371] 4D1-2
l(1)G0420[G0420] 5C3-8
l(1)G0365[G0365] 5E1-2
l(1)G0159[G0159] 5F5-6
l(1)G0140[G0140] ? -- multi-insert line: 6A, 13E14-F1
l(1)G0149[G0149] 6B
l(1)G0148[G0148] 6C1-2
l(1)G0255[G0255] 6C1-7
l(1)G0173[G0173] ? -- multi-insert line: 6E4-5, 13D1-2
l(1)G0011[G0011] semi-lethal 6F1-2
l(1)G0157[G0157] 6F1-2
l(1)G0281[G0281] 7D3-5
l(1)G0379[G0379] 7D20-22
l(1)G0356[G0356] 7E5-6
l(1)G0166[G0166] 7E5-10
l(1)G0424[G0424] 7E5-11
l(1)G0404[G0404] 8B1-4
l(1)G0415[G0415] 8B3-7
l(1)G0417[G0417] ? -- multi-insert line: 8B3-8, 14A
l(1)G0397[G0397] 8D8-12
l(1)G0320[G0320] 8E3-11
l(1)G0232[G0232] 8F3-7
l(1)G0350[G0350] 8F6-7
l(1)G0230[G0230] 9B1-8
l(1)G0289[G0289] 9C
l(1)G0388[G0388] 9E1-2
l(1)G0098[G0098] 9E1-4
l(1)G0351[G0351] 9E1-4
l(1)G0419[G0419] 9E1-4
l(1)G0436[G0436] 9E1-4
l(1)G0238[G0238] semi-lethal 9E3-4
l(1)G0391[G0391] 9E3-4
l(1)G0242[G0242] 9E3-6
l(1)G0247[G0247] 9E7-8
l(1)G0190[G0190] 10B1-2
l(1)G0342[G0342] 10B8-11
l(1)G0444[G0444] semi-lethal 10C
l(1)G0407[G0407] semi-lethal 10E
l(1)G0102[G0102] 10E1-4
l(1)G0292[G0292] 10E1-4
l(1)G0411[G0411] semi-lethal 10E1-4
l(1)G0361[G0361] 11C
l(1)G0429[G0429] 11E1-4
l(1)G0182[G0182] 12A1-2
l(1)G0416[G0416] 12E1-2
l(1)G0392[G0392] 12F1-5
l(1)G0160[G0160] 13E1-4
l(1)G0437[G0437] 13E14-F2
l(1)G0414[G0414] 13E14-F4
l(1)G0438[G0438] 13F1-4
l(1)G0369[G0369] 13F1-11
l(1)G0430[G0430] 14B5-8
l(1)G0382[G0382] 14B7-10
l(1)G0006[G0006] 15A1-5
l(1)G0213[G0213] 15E, 15F
l(1)G0316[G0316] 16B1-4
l(1)G0222[G0222] 16B6-11
l(1)G0358[G0358] 17C
l(1)G0381[G0381] 18D5-8
l(1)G0264[G0264] 18E
l(1)G0321[G0321] semi-lethal 18E
l(1)G0367[G0367] 18E
l(1)G0405[G0405] 18E
l(1)G0441[G0441] 18E
l(1)G0218[G0218] 18E1-2
l(1)G0353[G0353] 18F1-2
l(1)G0285[G0285] 19E
l(1)G0393[G0393] 20AB
New Transposon insertions (from multi-insert lines)
P{lacW}G0378a 1B7-10
P{lacW}G0378b 4E1-2
P{lacW}G0445a 3A1-4
P{lacW}G0445b 4C15-D2
P{lacW}G0152a 3A1-4
P{lacW}G0152b 7E9-11
P{lacW}G0400a 3A1-4
P{lacW}G0400b 8F
P{lacW}G0380a 3A1-4
P{lacW}G0380b 9E3-8
P{lacW}G0175a 3A1-4
P{lacW}G0175b 10F
P{lacW}G0434a 3A1-4
P{lacW}G0434b 17C5-7
P{lacW}G0375a 3A3-4
P{lacW}G0375b 14B3-4
P{lacW}G0140a 6A
P{lacW}G0140b 13E14-F1
P{lacW}G0173a 6E4-5
P{lacW}G0173b 13D1-2
P{lacW}G0417a 8B3-8
P{lacW}G0417b 14A
P{lacW}G0213a 15E
P{lacW}G0213b 15F
