From crosby@morgan Mon Dec 1 11:37:24 1997 Date: Mon, 1 Dec 1997 11:37:16 -0500 From: crosby@morgan (Madeline Crosby) To: perrimon@XXXX Subject: GAL4 construct Cc: crosby@morgan X-Sun-Charset: US-ASCII Content-Length: 358 Norbert, FlyBase needs a bit more information concerning the GAL4 construct or insertion called 'rhlGAL4' in Axelrod, et al., 1996, Science 271:1826 . Is this an insertion of P{GawB}? A GAL4 construct? If so, with regulatory sequences from what gene? To what does 'rhl' refer? Thanks for any enlightenment you can provide! \--Lynn Crosby FlyBase From noll@XXXX Wed Dec 3 14:26:15 1997 Date: Wed, 3 Dec 1997 14:26:15 -0500 Mime-Version: 1.0 To: crosby@XXXX From: noll@XXXX (Beth Noll) Subject: rhl gal4 Lynn, The rhl-gal4 is an pGawB insertion that was mapped by in situ to polytenes to the region where rhomboid lies. Based upon this, and the fact that it is expressed in the same pattern as rho, it was given the name 'rhomboid-like gal4' or 'rhl-gal4'. It is a viable insertion, and so it has likely not landed in the rhomboid gene itself, so we couched our naming of it to reflect that it's near rho, and is expressed like rho. I hope this info. is what you need. BN Elizabeth Noll Harvard Medical School-HHMI Department of Genetics Perrimon Lab 200 Longwood Ave. Boston, MA 02115 ph: (617)432-7571 fax: 432-7688 noll@XXXX