lastname : Van Doren
givenname : Mark
institution : Lehmann Lab NYU Medical Center Skirball Institute
email : vandoren@XXXX
FBgu ID : FBgu0000014
date_updated:
Oct 1 1996  4:35:23:260PM 
transposon:
PGAL4-nos.UTR
vector:
pPCaSpeR-4
regulatory_gene:
nanos promoter and 5' and 3' UTR's
insert_size:
5.1 kb
known_reg:
nanos promoter and 5' and 3' UTR's
vector_comments:
An identical construct is available using GAL4-VP16 in place of GAL4. GAL4-VP16 is a much more potent transcriptional activator that GAL4 (too good for some applications).
visualization:
via PUAS-lacZ.B and PUAS-GFPS65T
expression:
The nanos promoter should induce maternal nurse cell expression, and
also zygotic germ cell expression after the end of embryogenesis. The nanos
5' and 3' untranslated regions should cause the GAL4 mRNA to be localized
to the posterior pole and to be translated only at the posterior pole. The
observed pattern of expression is consistent with this in that GAL4
activity is found preferentially in the pole cells. There is also a
transient activity found in the posterior endoderm primordium. Expression
of lacZ and GFP has been observed in the primordial germ cells (pole cells)
from stg 8 (germ band extending) throughout embryogenesis and into larval
stages. GAL4 activity also appears to be present in the germ cells of the
male testes, but does not appear present in the nurse cells.
There appears to be consistent 'background' staining later in
development beginning after stg 12, and this is most likely due to aberrant
zygotic expression from the truncated nanos promoter as it is seen in
several lines.
reporter_comments:
An identical transposon was generated using GAL4-VP16 in place of GAL4.
GAL4-VP16 is a much more potent activator than GAL4 (too good for some
applications).
insertion_symbol:
PGAL4-nos.UTRB5-1
chromosome:
3
insertion_property:
lethal
insertion_symbol:
PGal4-nos.UTRB13-1
chromosome:
3
insertion_property:
viable