From rd120@XXXX Wed Nov 1 09:35:39 1995 From: Rachel Drysdale (Genetics) <rd120@XXXX> Date: Wed, 1 Nov 95 09:35:34 GMT To: karin.ekstrom@XXXX Subject: helping FlyBase Cc: rd120@XXXX Content-Length: 1872 Hi Karen, As you know, we are doing some work on your stock list for FlyBase, and I have a question about the Df(1)RA67 stock: U-#18350 Df(1)RA67 / FM7, y[31d] sc[8] w[a] sn[X2] v[Of] g[4] B[1] / y[+] Ymal[106] Breakpts: ?; 20B-D Comments: Center: Umea Stock Center Contact: Karin Ekstrom karin.ekstrom@XXXX Do you know where the breakpoint data 'Breakpts: ?; 20B-D' came from, or what it might mean? I ask because Michael Costa, then of the Weischaus lab, has published an allele they call fog[RA67] and when asked about whether it may be Df(1)RA67, he said >The RA67 allele of fog mentioned in my Cell paper is at least derived from >the the stock called Df(1)RA67 and quite possibly identical to it. When I >tested the Df(1)RA67 strain that Eric Wieschaus had in the lab, I found >that it complemented all mutations tested on both the proximal and distal >side of fog. Therefore, I started calling it fog[RA67] since there was no >genetic evidence that our allele was a deficiency. I never checked >cytologically for a deletion in this strain and never found any molecular >evidence of a deletion. Len Kelly has also found that his stock labelled >Df(1)RA67 also behaves genetically as a simple fog mutation, and it is not >clear whether Eric got his allele from Len or the Umea stock center. So you see it is not QUITE clear that they are the same thing, and we would like to be sure! Many thanks for your help, best wishes, Rachel \-------------------------------------------------------------- Rachel Drysdale. FlyBase (Cambridge), Department of Genetics, University of Cambridge, Downing Street, email: rd120@XXXX Cambridge, CB2 3EH, Ph : 01223-333963 UK. FAX: 01223-333992 \-------------------------------------------------------------- From Karin.Ekstrom@XXXX Thu Nov 2 11:29:46 1995 Date: Thu, 2 Nov 1995 12:24:43 +0100 X-Sender: Karin.Ekstrom@XXXX Mime-Version: 1.0 Content-Type: text/plain; charset='us-ascii' To: rd120@XXXX From: Karin.Ekstrom@XXXX v (Karin Ekstr\vm) Subject: X-Mailer: <PC Eudora Version 1.4> Content-Length: 424 Dear Rachel, About stock 18350.Df(1)RA67/ FM7, we received that stock from George Miklos Canberra October -87, with the following information Df; 114[-]-EA41[-]. I did send a copy to Len Kelly in November-88. He told me that he was already holding a copy of the strain but it was not behaving genetically as the original Df(1)RA67. That is all information I have. We have never been testing the stock. Best wishes, Karin
