Walldorf, U., Richter, S., Ryseck, R.P., Steller, H., Edstrom, J.E., Bautz, E.K.F., Hovemann, B. (1984). Cloning of heat-shock locus 93D from Drosophila melanogaster.  EMBO J. 3(3): 2499--2504.

FBrf0040775

Research paper

Using the microcloning approach a number of recombinant lambda phages carrying DNA from the 93D region have been isolated. Screening genomic libraries, cloned in phage lambda or cosmid vectors, with this isolated DNA yielded a series of overlapping DNA fragments from the region 93D6-7 as shown by in situ hybridization to polytene chromosomes. In vitro 32P-labelled nuclear RNA prepared from heat-shocked third instar larvae hybridized specifically to one fragment within 85 kb of cloned DNA. The region which is specifically transcribed after heat shock could be defined to a cluster of internally-repetitive DNA and its neighbouring proximal sequences. Over a sequence of 10-12 kb in length the DNA is cut into repeat units of approximately 280 nucleotides by the restriction endonuclease TaqI. The TaqI repeat sequences are unique in the Drosophila genome.

PMC557719 (PMC) (EuropePMC)