pWALIUM20 allows cloning of short hairpins as annealed synthetic oligos, targeted against specific genes via the production of short hairpin microRNAs; contains white as a selectable marker; the hsp70 basal promoter; a multiple cloning site (MCS) for cloning shRNAs in a mir-1 scaffold; a ftz 3'UTR intron followed by a SV40 3'UTR to facilitate shRNA processing and export from the nucleus; two pentamers of UAS, one of which is flanked by loxP sites and can thus be excised using the P1cre recombinase to generate a 5xUAS derivative; two gypsy insulator sequences flanking the transcription unit to enhance hairpin DNA transcription; an attB sequence to allow phiC31:int-targeted integration at genomic attP landing sites.
10.540
'WALIUM' stands for White-AttB-Loxp-Intron-UAS-MCS. See TRiP(http://fgr.hms.harvard.edu/trip-plasmid-vector-sets) for downloadable maps, sequences and cloning protocols. pWALIUM20 triggers effective RNAi knockdown and corresponding phenotypes in somatic tissue (stronger than pWALIUM10) and also works well in germline (not as strong as pWALIUM22).