FB2024_03 , released June 25, 2024
Recombinant Construct: pVALIUM1
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General Information
Symbol
pVALIUM1
FlyBase ID
FBmc0003033
Feature type
engineered_plasmid
Component Allele(s)
Size
6.742
Expression Data
Associated insertion(s)
0 available
Molecular map
Description and Uses
Description

pVALIUM1 allows cloning of a hairpin product downstream of UAS sequences for GAL4-inducible expression; contains v+t1.8 as a selectable marker; contains two introns: a white intron, located between the inverted DNA repeats, and a ftz intron between the hairpin product and the SV40 polyA tail to facilitate hairpin-RNA processing and export from the nucleus; two pentamers of UAS sites, one of which is flanked by loxP sites and can thus be excised using the P1cre recombinase to generate a 5xUAS derivative; an attB sequence to allow phiC31:int-targeted integration at genomic attP landing sites.

(Ni et al., 2008)
Construct components
Component
Use(s)
Regulatory region(s)
UAS
Also carries
attB
loxP
Marker(s)
v+t1.8
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
GU931383
(bases )
Segments and Size
Total Size

6.742

(Transgenic RNAi Project, 2017)
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Component Allele
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
Comments

Optimization of pVALIUM1, to eventually produce pVALIUM10, is described. The following parameters were tested: the directionality of the hairpin sequences, the choice of the basal promotor, the number and nature of introns, cloning vs. the recombination method, and the presence of insulator sequences.

(Ni et al., 2009)

See TRiP(http://fgr.hms.harvard.edu/trip-plasmid-vector-sets) for downloadable maps, sequences and cloning protocols.

(Transgenic RNAi Project, 2017)

'VALIUM' stands for Vermilion-AttB-Loxp-Intron-UAS-MCS.

(Ni et al., 2008)
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
VALIUM1
(Ni et al., 2009)
pVALIUM1
(FlyBase, 1996-)
Secondary FlyBase IDs
    References (6)