construct_comment: Contains P1\loxP site within the polylinker (between KpnI and EcoRI); pP{CaSpeR4-lo+} and pP{CaSpeR4-lo-} differ in orientation of loxP site.For use with pUNIΔ shuttle construct; allows insertion of segment of interest via cre-loxP recombination.
construct_comment: Contains P1\loxP site within the polylinker (between KpnI and EcoRI); pP{CaSpeR4-lo+} and pP{CaSpeR4-lo-} differ in orientation of loxP site.For use with pUNIΔ shuttle construct; allows insertion of segment of interest via cre-loxP recombination.
construct_comment: Cloning sites based on output of NEBcutter V2.0.