FB2024_03 , released June 25, 2024
Recombinant Construct: pP{CaSpeR-lacZ}
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General Information
Symbol
pP{CaSpeR-lacZ}
FlyBase ID
FBmc0000180
Feature type
engineered_plasmid
Component Allele(s)
Size
Expression Data
Associated insertion(s)
0 available
Molecular map
Description and Uses
Construct components
Sequence Data
Sequence (FB)
FlyBase-compiled
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
X81643
(bases )
Segments and Size
Total Size
Left end

EcoRI (inclusive)

(Thummel and Pirrotta, 1991)

P5\' (inclusive) (stated as revision)

(FlyBase, 1992-)
Right end

EcoRI

(Thummel and Pirrotta, 1991)

P5\' (exclusive) (stated as revision)

(FlyBase, 1992-)
 
Segments
Num
Orientation
Symbol
1
+
pW8mcs-St
1
+
CaSpeR_P5'(P5',Sp)
2
+
pCaSpeR-lacZ-mcs
2
+
pW8mcs-St
3
+
CaSpeR-lacZ_P3'(r,P3')
4
+
pCar1-P
Reference
(FlyBase, 1992-, Thummel and Pirrotta, 1991)
Phenotypic Data
Component Allele
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
Comments

construct_comment: Allows cloning and testing of regulatory sequences in front of lacZ reporter gene.

(Thummel and Pirrotta, 1991)

construct_comment: NOTE: A map of this construct can be viewed on the Web page "P-element vector maps" at http://www-hhmi.genetics.utah.edu/thummel/pelement.html.

(Thummel, 1996.8.8)

report comment: CAUTION: GenBank entry X81643 | 551429 | 29 Sep 1994 appears to be in error; relative to other pP{CaSpeR} vectors, 60bp missing after coordinate 8505. See also report for component segment w+mC4.1(Sp) : sequence discrepancies.

(Thummel and Pirrotta, 1991)
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
pCaSpeR_hs43_lacZ
(Thummel and Pirrotta, 1991)
pP{CaSpeR-lacZ}
(FlyBase, 1996-, Pirrotta, 1994.9.14)
Secondary FlyBase IDs
    References (9)