FlyBase Recombinant Construct Report: pP{CaSpeR-hs}

FB2024_03 , released June 25, 2024

FB2024_03 , released June 25, 2024

Recombinant Construct: pP{CaSpeR-hs}

General Information

Symbol

pP{CaSpeR-hs}

FlyBase ID

FBmc0000179

Feature type

engineered_plasmid

Component Allele(s)

Size

Expression Data

Associated insertion(s)

Molecular map

Description and Uses

Construct components

Sequence Data

Sequence (FB)

FlyBase-compiled

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

(bases 1-8780 )

Segments and Size

Total Size

Left end

EcoRI (inclusive)

(Thummel and Pirrotta, 1991)

StuI

(Thummel, 1996.8.8)

P5\' (inclusive) (stated as revision)

(FlyBase, 1992-)

Right end

EcoRI

(Thummel and Pirrotta, 1991)

StuI (inclusive)

(Thummel, 1996.8.8)

P5\' (exclusive) (stated as revision)

(FlyBase, 1992-)

Segments

Num

Orientation

Symbol

1

+

CaSpeR-hs_P5'(P5',R1)

2

+

3

+

CaSpeR-hs_P3'(St,P3')

4

+

Reference

(Thummel, 1996.8.8, Thummel and Pirrotta, 1991)

Phenotypic Data

Component Allele

Phenotypic Data

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

(Thummel and Pirrotta, 1991)

descendant(s)

(Thummel and Pirrotta, 1991)

(Harden et al., 1995)

(Harden et al., 1995)

(Kerber et al., 1996)

(Kerber et al., 1996)

(Kerber et al., 1996)

(Kodjabachian et al., 1998)

(van de Wetering et al., 1997)

(Fisk and Thummel, 1998)

P{Hsp70.tTA.Ba}

(Bello et al., 1998)

(Ruohola-Baker et al., 1993)

(Ruohola-Baker et al., 1993)

(Murphy and Montell, 1996)

(Murphy and Montell, 1996)

(White et al., 1996)

(White et al., 1996)

(Yu et al., 1996)

P{hs-yCDC42.V12}

(Murphy and Montell, 1996)

(Harden et al., 1995)

(Fauvarque et al., 1995)

(Fauvarque et al., 1995)

P{βTub85D-Scer\SCEI.nls}

(Bellaiche et al., 1999)

(Lamka et al., 1992)

(Lamka et al., 1992)

(Mottes and Iverson, 1995)

(Calvi and Gelbart, 1994)

(Parks, 2004.5.6)

pP{Express-FRT.2.1}

(Parks, 2004.5.6)

pP{Express-FRT.2.2}

(Parks, 2004.5.6)

pP{Express-FRT.2.3}

(Parks, 2004.5.6)

pP{GAL4::Pc-Hsp70}

(Muller, 1995)

pP{GAL4-ninaE.GMR}

(Freeman, 1997.3.15)

(Hay et al., 1994)

(Axelrod et al., 1996)

(Knoblich and Lehner, 1993)

(Knoblich and Lehner, 1993)

(Fuse et al., 1994)

(Halder et al., 1995)

(Han et al., 2000)

(Ingham, 1993)

(Harrison et al., 1995)

pP{hsp70-DmcycE}

(Richardson et al., 1995)

pP{Hsp70-Draf.gof}

(Brand and Perrimon, 1994)

pP{Hsp70-Hraf.gof}

(Brand and Perrimon, 1994)

(Ingham et al., 1991)

(Schweitzer et al., 1995)

(Ellis et al., 1994)

(Schweitzer et al., 1995)

(Rebay and Rubin, 1995)

(Rebay and Rubin, 1995)

(Fuse et al., 1994)

(Lee et al., 1996)

(Xu and Rubin, 1993)

(Bello et al., 1998)

(Brand and Perrimon, 1993)

pP{vg(int2.1)-ap}

(Williams et al., 1994)

(Xu and Rubin, 1993)

Comments

construct_comment: Allows heat-induced expression of inserted open-reading-frame.

(Thummel and Pirrotta, 1991)

report comment: Compiled sequence is an alternate view: in opposite orientation relative to (sequence entry U59056 | 1432064 | 18 Jul 1996); some sequence discrepancies.

(Thummel, 1996.8.8)

construct_comment: NOTE: A map of this construct can be viewed on the Web page "P-element vector maps" at http://www-hhmi.genetics.utah.edu/thummel/pelement.html.

(Thummel, 1996.8.8)

construct_comment: Additional reference: "P-element vector maps" Web page maintained by Carl Thummel.

(Thummel, 1996.8.8)

report comment: Compiled sequence is an alternate view: in opposite orientation relative to sequence entry. Included as non-attributed correction.

(Thummel and Pirrotta, 1991)

Stocks (0)

Stocks containing insertion(s) of this recombinant construct

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

pCaSpeR-hs

(Thummel and Pirrotta, 1991)

pP{CaSpeR-hs}

(FlyBase, 1996-, Thummel, 1996.5.23)

Secondary FlyBase IDs

References (42)

Report Sections