Staged larvae were infested at the L2 stage (48-56 h AEL) for 2 h at 20oC with 20 female wasps (L. boulardi) per 100 Drosophila larvae. Following infestation, the larvae were raised at 25oC until the wandering L3 stage, where 20 female larvae were collected and bled in Schneider medium complemented with N-phenylthiourea on ice. Both circulating and resident pools of hemocytes were collected by first puncturing the cuticle to release the circulating hemocytes, then by scrapping the cuticle with fine forceps to release the resident hemocytes (FBrf0230406). After bleeding, the hemolymph was filtered on a 100um-mesh to remove cell aggregates and wasp eggs.