A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GSV1}. The P{GSV1} construct construct carries a w+mC mini-white visible marker; it carries two sets Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator plus a core promoter, one adjacent to each terminus and oriented to direct transcription outward. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity.
Mobilization of the P-element occurred in animals carrying P{GSV1} on a second chromosome CyO balancer chromosome and P{Δ2-3}99B, a stable source of P transposase, on a marked third chromosome. Stable homozygous or balanced stocks were created.
For the initial collection, 613 stable lines were crossed to 4 different GAL4-expressing lines; F1 individuals carrying both a GAL4 transgene and a P{GSV1} insert were screened for lethality and visible phenotypes. GAL4 expression patterns were observed using the P{UAS-GFP.S65T} reporter construct. For a subset of 170 lines, the identity of the induced transcript(s) was determined by RT-PCR, using vector-specific and oligo-dT primers.
