A set of transgenic insertion stocks derived by TE mobilization using the "piggyBac mutator" construct PBac{GAL4D,EYFP}. The dataset consists primarily of insertions on the third chromosomes that also carry proximal Scer\FRT sites, to allow generation of germ-line clones. The PBac{GAL4D,EYFP} construct carries the yellow fluorescent marker Avic\GFPEYFP.3xP3 (which can be distinguished from other GFP-based markers) and a modified Scer\GAL4 driver/reporter gene.
Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection; some lines were mapped by the GDP.
A PBac{GAL4D,EYFP} element located on the X chromosome was mobilized by a piggyBac transposase source, Tni\piggyBac\TαTub84B.PH, integrated on the second chromosome. Third chromosomes carrying proximal Scer\FRT sites were used, to facilitate recovery of insertions useful for generation of germ-line clones. 798 third chromosome insertions were chosen for futher analysis.
Markers in stocks (as originally submitted to the BDSC):w*, cn1 bw1; P{FRT(whs)}2A P{neoFRT}82B.
Genomic sequences flanking recessive lethal insertions were determined by sequencing of inverse-PCR products.
Flanking genomic sequences were obtained by sequencing of inverse PCR products, and were mapped by alignment to the genomic sequence. A total of 266 PBac{GAL4D,EYFP} lines were selected for inclusion in the BDGP Gene Disruption Project 2004 collection.
One of several datasets using a Tni\piggyBac-based construct rather than a P-element construct that were included in the GDP collection, in order to broaden the spectrum of insertion sites.
