Set of Y;2 translocation stocks designed to allow generation of aneuploid animal by crossing two members of the set with breakpoints flanking the region of interest. For most regions, animals carrying both the duplication and the deficiency of the region between the autosomal breakpoints can be recovered and unambiguously identified.
Males carrying Dp(1;Y)BSYy+ were used as progenitors: the Y chromosome carried the dominant marker Bar-Stone on the tip of its long arm and the wild-type allele of yellow at the terminus of its short arm). Males were X-irradiated and crossed to attached-XY/X females carrying recessively marked autosomes. Atypical segregation of the autosomal markers in subsequent crosses allowed identification of Y:autosome translocations; survival of aneuploid classes allowed classification of reciprocal translocations. Established in stocks with attached-X females and an appropriate autosomal balancer. Established 233 second chromosome lines; 166 of these determined to be simple deficiencies; 164 were mapped cytologically.
