FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Nlg2, tsr
FlyBase ID
FBig0000123087
Interaction Network
Interactions Browser links
Nlg2 network
tsr network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0257922-2.coIP.MS
Reference
Sun et al., 2023
Description
physical association
Assay
Collection
Source/Stage
head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Nlg2 

Nlg2

bait

GFP tag

tsr 

Cofilin

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Sun et al., 2023)
FBrf0257922-4.coIP.WB
Reference
Sun et al., 2023
Description
physical association
Assay
Collection
Source/Stage
head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Nlg2 

Nlg2

bait

HA tag

tsr 

Cofilin

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Sun et al., 2023)
FBrf0257922-5.coIP.WB
Reference
Sun et al., 2023
Description
physical association
Assay
Collection
Source/Stage
head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
tsr 

Cofilin

bait

GFP tag

Nlg2 

Nlg2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Sun et al., 2023)
FBrf0257922-8.PD.MS
Reference
Sun et al., 2023
Description
physical association
Assay
Collection
Source/Stage
head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Nlg2 

Nlg2

bait

GST tag

Nlg2 
Nlg2 
tsr 

Cofilin

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Nlg2 
intracellular domain
sufficient binding region
aa 989-1248

coordinates relative to Nlg2-PA

Nlg2 
extracellular domain
sufficient binding region
aa 1-965

coordinates relative to Nlg2-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type adult head extract.

(Sun et al., 2023)
External Crossreferences and Linkouts ( 0 )
References (1)