FB2024_03 , released June 25, 2024
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
Nlg2, Rack1
FlyBase ID
FBig0000123012
Interaction Network
Interactions Browser links
Nlg2 network
Rack1 network
esyN Network Diagram
Show neighbor-neighbor interactions:
Select Layout:
Legend:
Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0257922-1.coIP.MS
Reference
Sun et al., 2023
Description
physical association
Assay
Collection
Source/Stage
head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Nlg2 

Nlg2

bait

GFP tag

Rack1 

Rack1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Sun et al., 2023)
FBrf0257922-3.coIP.WB
Reference
Sun et al., 2023
Description
physical association
Assay
Collection
Source/Stage
head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Nlg2 

Nlg2

bait

HA tag

Rack1 

Rack1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Sun et al., 2023)
FBrf0257922-7.PD.MS
Reference
Sun et al., 2023
Description
physical association
Assay
Collection
Source/Stage
head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Nlg2 

Nlg2

bait

GST tag

Nlg2 
Rack1 

Rack1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Nlg2 
intracellular domain
sufficient binding region
aa 989-1248

coordinates relative to Nlg2-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type adult head extract.

(Sun et al., 2023)
FBrf0257922-9.coIP.WB
Reference
Sun et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Nlg2 

Nlg2

bait

Strep tag

Nlg2 
Rack1 

Rack1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Nlg2 
C terminal domain
sufficient binding region
aa 685-1248

coordinates relative to Nlg2-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait and prey synthesized.

(Sun et al., 2023)
External Crossreferences and Linkouts ( 0 )
References (1)