FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
TH1, Nelf-A
FlyBase ID
FBig0000123010
Interaction Network
Interactions Browser links
TH1 network
Nelf-A network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0257712-14.coIP.MW
Reference
Dollinger et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
TH1 

NelfD

bait

FLAG tag

Nelf-A 

NelfA

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait and prey produced as a recombinant fusion protein in a baculovirus and insect cell system.

(Dollinger et al., 2023)

The NELF complex was tandem purified with Nelf-E affinity purification followed by immunoprecipitation with TH1-anti-FLAG antibody. Nelf-A, NELF-B, TH1, and Nelf-E captured as 3 pairwise interactions.

(Dollinger et al., 2023)
External Crossreferences and Linkouts ( 0 )
References (1)