FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Nuak, l(2)efl
FlyBase ID
FBig0000122992
Interaction Network
Interactions Browser links
Nuak network
l(2)efl network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0257934-3.Y2H
Reference
Zhao et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Nuak 

Nuak

bait

fused to GAL4 DNA-binding domain

l(2)efl 

CryAB

prey

fused to GAL4 activation domain

l(2)efl 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
l(2)efl 
interaction domain
sufficient binding region
aa 78-156
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Zhao et al., 2023)
FBrf0257934-4.PLA
Reference
Zhao et al., 2023
Description
physical association
Assay
Collection
Source/Stage
organism / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Nuak 

Nuak

bait
l(2)efl 

CryAB

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval wing discs of transgenic fly line; bait produced from transgenic construct; prey produced from transgenic construct.

(Zhao et al., 2023)
External Crossreferences and Linkouts ( 0 )
References (1)