FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000122349
Interaction Network
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dunk network
scra network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0257275-1.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dunk 

Dunk

bait

fused to GAL4 DNA-binding domain

scra 

Scraps

prey

fused to GAL4 activation domain

scra 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
scra 
C-terminal domain
sufficient binding region
aa 630-1212

coordinates relative to scra-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from universal cDNA expression library).

Two-hybrid system: yeast GAL4/GAL4-AD; Universal (embryonic, larval and adult) cDNA library was used.

FBrf0257275-2.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dunk 

Dunk

bait

fused to GAL4 DNA-binding domain

scra 

Scraps

prey

fused to GAL4 activation domain

scra 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
scra 
PH domain
necessary binding region
aa 1000-1212

coordinates relative to scra-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

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References (1)