FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Mst27D, Nup358
FlyBase ID
FBig0000122074
Interaction Network
Interactions Browser links
Mst27D network
Nup358 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0257084-1.coIP.MS
Reference
Li et al., 2023
Description
physical association
Assay
Collection
Source/Stage
testis / malethird instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mst27D 

Mst27D

bait

GFP tag

Nup358 

Nup358

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was late larval and early pupal testes of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Li et al., 2023)
FBrf0257084-2.coIP.WB
Reference
Li et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Nup358 

Nup358

bait

mCherry tag

Nup358 
Mst27D 

Mst27D

prey

GFP tag

Mst27D 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Mst27D 
C terminal domain
sufficient binding region
aa 151-424

coordinates relative to AA-PA

Nup358 
C terminal domain
necessary binding region
aa 2342-2718

coordinates relative to AA-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Li et al., 2023)
External Crossreferences and Linkouts ( 0 )
References (1)