FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
E(z), Sirt1
FlyBase ID
FBig0000121156
Interaction Network
Interactions Browser links
E(z) network
Sirt1 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0180067-11.PD.A
Reference
Furuyama et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
E(z) 

E(Z)

bait

GST tag

Sirt1 

Sir2

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Furuyama et al., 2004)
FBrf0180067-3.CH.WB
Reference
Furuyama et al., 2004
Description
physical association
Assay
Collection
Source/Stage
organism / larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Sirt1 

Sir2

neutral component
Sirt1 

Sir2

neutral component
Sirt1 

Sir2

neutral component
E(z) 

E(Z)

neutral component
E(z) 

E(Z)

neutral component
E(z) 

E(Z)

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval extracts; proteins produced from endogenous genes.

(Furuyama et al., 2004)
FBrf0180067-4.coIP.WB
Reference
Furuyama et al., 2004
Description
physical association
Assay
Collection
Source/Stage
organism / larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
E(z) 

E(Z)

bait
Sirt1 

Sir2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval extracts; bait produced from endogenous gene; prey produced from endogenous gene.

(Furuyama et al., 2004)
FBrf0180067-5.coIP.WB
Reference
Furuyama et al., 2004
Description
physical association
Assay
Collection
Source/Stage
organism / larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Sirt1 

Sir2

bait
E(z) 

E(Z)

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval extracts; bait produced from endogenous gene; prey produced from endogenous gene.

(Furuyama et al., 2004)
FBrf0180067-8.PD.A
Reference
Furuyama et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Sirt1 

Sir2

bait

GST tag

Sirt1 
Sirt1 
Sirt1 
E(z) 

E(Z)

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Sirt1 
interaction domain residues 209-362
sufficient binding region
aa 209-362

coordinates relative to Sirt1-PA

Sirt1 
interaction domain residues 362-570
sufficient binding region
aa 362-570

coordinates relative to Sirt1-PA

Sirt1 
interaction domain residues 570-705
sufficient binding region
aa 570-705

coordinates relative to Sirt1-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Furuyama et al., 2004)
External Crossreferences and Linkouts ( 0 )
References (1)