FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
E(bx), EcR
FlyBase ID
FBig0000120935
Interaction Network
Interactions Browser links
E(bx) network
EcR network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0190536-1a.PD.A
Reference
Badenhorst et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
E(bx) 

NURF

bait

Flag tag

EcR 

EcR

prey

[35]S label

EcR 
EcR 
EcR 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
EcR 
Af2 domain
sufficient binding region
aa 362-689

coordinates relative to EcR-PA.

EcR 
AF2 domain
necessary binding region
aa 651-689

coordinates relative to EcR-PA

EcR 
AF2 domain residue 645
mutation disrupting interaction
aa 645

F645A, coordinates relative to EcR-PA.

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Flag-tagged NURF complex binds to aa 362-689 of EcR. Recombinant NURF complex included Flag-E(bx), HA-Iswi, Myc-Caf1-55 and AU1-Nurf-38. It is unclear which of these complex subunits directly contacts EcR.

(Badenhorst et al., 2005)

Interaction in vitro; bait was NURF complex produced in a baculovirus and Sf9 cell system; prey produced and labeled by in vitro translation.

(Badenhorst et al., 2005)
External Crossreferences and Linkouts ( 0 )
References (1)