FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
uno, SA2
FlyBase ID
FBig0000120601
Interaction Network
Interactions Browser links
uno network
SA2 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0246854-2.coIP.MS
Reference
Weber et al., 2020
Description
physical association
Assay
Collection
Source/Stage
testis / wandering third instar larval stagepupal stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
SA2 

snm

bait

GFP tag

uno 

uno

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was testes of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Weber et al., 2020)
FBrf0254845-5.coIP.WB
Reference
Kabakci et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
uno 

uno

bait

EGFP tag

SA2 

snm

prey

mCherry tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Kabakci et al., 2022)
FBrf0255412-1.coIP.WB
Reference
Kabakci et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
uno 

UNO

bait

EGFP tag

uno 
SA2 

SNM

prey

mCherry tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
uno 
C terminal domain
sufficient binding region
aa 241-417

coordinates relative to uno-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Kabakci et al., 2022)

In figure 2F the experiment was carried out in cells also co-expressing SA2, mod(mdg4), and tef.

(Kabakci et al., 2022)
FBrf0255412-17.PD.CH.MALS
Reference
Kabakci et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
uno 

UNO

bait

Streptactin tag

uno 
SA2 

SNM

prey

MBP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
uno 
C terminal domain
sufficient binding region
aa 281-417
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as recombinant fusion proteins in baculovirus system.

(Kabakci et al., 2022)
FBrf0255412-19.EMSA.TV
Reference
Kabakci et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
uno 

UNO

neutral component

Streptactin tag

uno 

UNO

neutral component

Streptactin tag

uno 
uno 

UNO

neutral component

Streptactin tag

uno 
SA2 

SNM

neutral component

MBP tag

SA2 

SNM

neutral component

MBP tag

SA2 

SNM

neutral component

MBP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
uno 
C terminal domain
sufficient binding region
aa 281-417
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The purified uno-SA2 complex was mixed with Cy5 labeled DNA. DNA-protein complexes observed by EMSA were used to deduce protein-protein interactions. Mutated basic amino acids in both SA2 and uno reduced binding in Fig4E. However, due to unspecified techniques, the experiment was left out.

(Kabakci et al., 2022)

Interaction in vitro; proteins produced as recombinant fusion proteins in baculovirus system.

(Kabakci et al., 2022)

uno and SA2 bind DNA cooperatively.

(Kabakci et al., 2022)
FBrf0255412-2.coIP.WB
Reference
Kabakci et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
uno 

UNO

bait

EGFP tag

uno 
uno 
SA2 

SNM

prey

mCherry tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
uno 
C terminal domain
sufficient binding region
aa 241-417

coordinates relative to uno-PA

uno 
N terminal domain
sufficient binding region
aa 1-78

coordinates relative to uno-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Kabakci et al., 2022)
FBrf0255412-20.coIP.WB
Reference
Kabakci et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
SA2 

SNM

bait

mCherry tag

uno 

UNO

prey

EGFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Kabakci et al., 2022)

Interaction of SA2 and uno requires mod(mdg4).

(Kabakci et al., 2022)
FBrf0255412-23.CL.MS
Reference
Kabakci et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
uno 

UNO

neutral component

Streptactin tag

uno 
uno 

UNO

neutral component

Streptactin tag

uno 
uno 

UNO

neutral component

Streptactin tag

SA2 

SNM

neutral component

MBP tag

SA2 

SNM

neutral component

MBP tag

SA2 

SNM

neutral component

MBP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
uno 
C terminal domain
sufficient binding region
aa 281-417
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as recombinant fusion proteins in baculovirus system.

(Kabakci et al., 2022)
FBrf0255412-9.coIP.WB
Reference
Kabakci et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
SA2 

SNM

bait

mCherry tag

uno 

UNO

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Kabakci et al., 2022)
External Crossreferences and Linkouts ( 0 )
References (3)