Source was ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
Source was nuclear extract of OSC cell line; bait produced from tagged transgenic construct; prey produced from endogenous gene.
I398A, L400A; within the context of SP-Ring+CTD domain aa 278-408; coordinates relative to Su(var)2-10-PJ
Y517A, Y517F, R520A, R526A; coordinates relative piwi-PA
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced by in vitro translation.
piwi Mid domain demonstrated the strongest interaction with Su(var)2-10 SP-RING+CTD domain, but was reduced with the CTD-2 mutation of Su(var)2-10 or mutation of amino acids in the piwi SIM binding site within the MID domain.
I398A, L400A; coordinates relative to Su(var)2-10-PJ
Source was ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from transgenic construct.
