FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
vas, Rm62
FlyBase ID
FBig0000120214
Interaction Network
Interactions Browser links
vas network
Rm62 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0243967-12.coIP.MS
Reference
Durdevic and Ephrussi, 2019
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
vas 

Vas

bait

GFP tag

Rm62 

Rm62

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Durdevic and Ephrussi, 2019)
FBrf0243967-6.coIP.WB
Reference
Durdevic and Ephrussi, 2019
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
vas 

Vas

bait

GFP tag

Rm62 

Rm62

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

A E400Q mutant form of vas that locks vas containing protein complexes was used in the IP assays.

(Durdevic and Ephrussi, 2019)

Source was ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Durdevic and Ephrussi, 2019)
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References (1)