FB2024_03 , released June 25, 2024
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
eIF4A, Mad
FlyBase ID
FBig0000120024
Interaction Network
Interactions Browser links
eIF4A network
Mad network
esyN Network Diagram
Show neighbor-neighbor interactions:
Select Layout:
Legend:
Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0192376-1.coIP.WB
Reference
Li and Li, 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
eIF4A 

eIF4A

bait

His tag

Mad 

Mad

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of 293T cell line; bait produced from transfected construct; prey produced from transfected construct.

(Li and Li, 2006)
FBrf0192376-3.coIP.WB
Reference
Li and Li, 2006
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mad 

Mad

bait
eIF4A 

eIF4A

prey
eIF4A 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
eIF4A 
interaction domain
mutation decreasing interaction
aa 321

R321H

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Li and Li, 2006)
FBrf0192376-4.coIP.WB
Reference
Li and Li, 2006
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
eIF4A 

eIF4A

bait
eIF4A 
Mad 

Mad

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
eIF4A 
interaction domain
mutation decreasing interaction
aa 321

R321H

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Li and Li, 2006)
FBrf0192376-5.PD.WB
Reference
Li and Li, 2006
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
eIF4A 

eIF4A

bait

His tag

eIF4A 
Mad 

Mad

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
eIF4A 
interaction domain
mutation decreasing interaction
aa 321

R321H

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.

(Li and Li, 2006)
External Crossreferences and Linkouts ( 0 )
References (1)