FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
per
FlyBase ID
FBig0000116162
Interaction Network
Interactions Browser links
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0207795-1.CH.MW
Reference
Hennig et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
per 

dPER

self
per 

dPER

self
per 
per 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
per 
PAS domains
sufficient binding region
aa 232-599
per 
PAS domains
mutation disrupting interaction
aa 482,560

W482E,M560D

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system.

(Hennig et al., 2009)

The fragment of per consisting of aa 232-599 elutes as a dimer in gel filtration.

(Hennig et al., 2009)
FBrf0207795-2.CS.MW
Reference
Hennig et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
per 

dPER

self
per 

dPER

self
per 
per 
per 
per 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
per 
PAS domains
sufficient binding region
aa 232-599

Kd=0.2uM

per 
PAS domains
mutation disrupting interaction
aa 482,560

W482E,M560D

per 
PAS domains
mutation decreasing interaction
aa 482aa 560

W482E, Kd=3.7uM

M560D, Kd=7.14uM

per 
PAS domains
necessary binding region
aa 539-599

For per fragment aa 232-538, Kd=256uM

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system.

(Hennig et al., 2009)
FBrf0207796-1.coIP.WB
Reference
Landskron et al., 2009
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
per 

per

bait

Myc tag

per 

per

prey

HA tag

per 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
per 
PAS domain residue 560
mutation disrupting interaction
aa 560

M560D

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

(Landskron et al., 2009)
FBrf0255496-2.coIP.WB
Reference
Giesecke et al., 2023
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
per 

per

bait

Myc tag

per 

per

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Giesecke et al., 2023)
External Crossreferences and Linkouts ( 0 )
References (3)