FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Ote, baf
FlyBase ID
FBig0000116076
Interaction Network
Interactions Browser links
Ote network
baf network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0207348-3.Y2H
Reference
Pinto et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ote 

Otefin

bait

fused to GAL4 DNA-binding domain

baf 

dBAF

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Pinto et al., 2008)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Pinto et al., 2008)
FBrf0257049-1.PD.MS
Reference
Emond-Fraser et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ote 

Ote

bait

GFP tag

baf 

Baf

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was fixed embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene;

(Emond-Fraser et al., 2023)
FBrf0257049-3.coIP.WB
Reference
Emond-Fraser et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
baf 

BAF

bait

Myc tag

Ote 

otefin

prey

GFP tag

Ote 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Ote 
interaction domain residues 50 and 54
mutation disrupting interaction
aa 50 and 54

S50D, S54D; coordinates relative to ote-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Emond-Fraser et al., 2023)

Phosphomimetic mutations of Ote at Ser50 amd Ser54 negatively regulates its interaction with baf. tws RNAi disrupts Ote-baf interaction.

(Emond-Fraser et al., 2023)
FBrf0257049-4.coIP.WB
Reference
Emond-Fraser et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Ote 

Ote

bait

GFP tag

Ote 
baf 

Baf

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Ote 
interaction domain residues 50 and 54
mutation disrupting interaction
aa 50 and 54

S50D, S54D within the context of aa 1-70; coordinates relative to ote-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Phosphomimetic mutations of Ote at Ser50 amd Ser54 negatively regulates its interaction with baf.

(Emond-Fraser et al., 2023)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Emond-Fraser et al., 2023)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)