FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Nup35, Nup154
FlyBase ID
FBig0000115853
Interaction Network
Interactions Browser links
Nup35 network
Nup154 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0219921-2a.PD.WB
Reference
Busayavalasa et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Nup35 

Nup53

bait

GST tag

Nup154 

Nup155

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type cell extract.

(Busayavalasa et al., 2012)
FBrf0219921-4a.PD.A
Reference
Busayavalasa et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Nup35 

Nup53

bait

GST tag

Nup154 

Nup155

prey

[35]S label

Nup154 
Nup154 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Nup154 
β-Propeller domain
sufficient binding region
aa 1-539

\'Nup155N\'

Nup154 
central region
sufficient binding region
aa 508-986

\'Nup155M\'

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Busayavalasa et al., 2012)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)