FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Pdp, Mad
FlyBase ID
FBig0000115806
Interaction Network
Interactions Browser links
Pdp network
Mad network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0190539-1.ENZ.WB
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Pdp 

PDP

enzyme

GST tag

Pdp 

PDP

enzyme

GST tag

Pdp 
Mad 

MAD

enzyme target

FLAG tag

Mad 

MAD

enzyme target

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0190539-2.coIP.WB
Reference
Chen et al., 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Pdp 

PDP

bait

V5 tag

Mad 

MAD

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Chen et al., 2006)
FBrf0190539-3.coIP.WB
Reference
Chen et al., 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Mad 

MAD

bait

FLAG tag

Pdp 

PDP

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Chen et al., 2006)
FBrf0190539-4.PD.WB
Reference
Chen et al., 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Pdp 

PDP

bait

GST tag

Mad 

MAD

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected S2 cell extract.

(Chen et al., 2006)

.

(Chen et al., 2006)

More phosphorylated Mad interacted with Pdp than did unphosphorylated Mad

(Chen et al., 2006)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)