Source was thoraces of transgenic fly line; bait produced from endogenous gene with inserted protein tag; prey produced from endogenous gene with inserted protein tag.
Source was adult thoraces of transgenic fly line; bait produced from endogenous gene with inserted protein tag; prey produced from endogenous gene.
Interaction in vitro; bait purified from transgenic adult thoracic extract; prey produced as a recombinant fusion protein in bacterial system.