FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000113101
Interaction Network
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sls network
cher network
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0236159-1.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sls 

Sls

bait

His tag

cher 

Cher

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was thoraces of transgenic fly line; bait produced from endogenous gene with inserted protein tag; prey produced from endogenous gene with inserted protein tag.

FBrf0236159-2.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cher 

Cher

bait

FLAG tag

sls 

Kettin

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult thoraces of transgenic fly line; bait produced from endogenous gene with inserted protein tag; prey produced from endogenous gene.

FBrf0236159-3.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sls 

Sls

bait

GFP tag

cher 

Cher

prey

GST tag

cher 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
cher 
Ig domains 19-22
sufficient binding region
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait purified from transgenic adult thoracic extract; prey produced as a recombinant fusion protein in bacterial system.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)