FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
corto, RpL12
FlyBase ID
FBig0000109060
Interaction Network
Interactions Browser links
corto network
RpL12 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0219702-1.PD.MS
Reference
Coléno-Costes et al., 2012
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
corto 

corto

bait

GST tag

corto 
RpL12 

RpL12

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
corto 
chromo domain
sufficient binding region
aa 127-203
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.

(Coléno-Costes et al., 2012)

Unvalidated interactors not curated.

(Coléno-Costes et al., 2012)
FBrf0219702-2.coIP.WB
Reference
Coléno-Costes et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
corto 

corto

bait

FLAG tag

corto 
corto 
RpL12 

RpL12

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
corto 
chromo domain
sufficient binding region
aa 127-203
corto 
chromo domain
necessary binding region
aa 1-203
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Coléno-Costes et al., 2012)
FBrf0219702-3.coIP.WB
Reference
Coléno-Costes et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
RpL12 

RpL12

bait

Myc tag

corto 

corto

prey

FLAG tag

corto 
corto 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
corto 
chromo domain
sufficient binding region
aa 127-203
corto 
chromo domain
necessary binding region
aa 1-203
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Coléno-Costes et al., 2012)
FBrf0219702-4.SPR
Reference
Coléno-Costes et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
corto 

corto

bait
corto 
RpL12 

RpL12

prey
RpL12 
RpL12 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
corto 
chromo domain
sufficient binding region
aa 127-203
RpL12 
N-terminal domain
sufficient binding region
aa 1-23
RpL12 
N-terminal domain residue 3
mutation disrupting interaction
aa 3

K3A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey peptide synthesized in vitro.

(Coléno-Costes et al., 2012)
FBrf0254298-1.coIP.WB
Reference
Grunchec et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
corto 

corto

bait

FLAG tag

corto 
RpL12 

uL11

prey

Myc tag

RpL12 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
corto 
CD domain
sufficient binding region
unspecified

coordinates relative to corto-PA

RpL12 
interaction domain residue 3
necessary binding region
aa 3

K3A or K3Y; coordinates relative to RpL12-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Grunchec et al., 2022)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)