FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
ChAT, Klp68D
FlyBase ID
FBig0000108963
Interaction Network
Interactions Browser links
ChAT network
Klp68D network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0191019-6.coIP.WB
Reference
Baqri et al., 2006
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ChAT 

ChAT

bait
Klp68D 

KLP68D

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Baqri et al., 2006)
FBrf0218568-1.coIP.WB
Reference
Sadananda et al., 2012
Description
physical association
Assay
Collection
Source/Stage
head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Klp68D 

KLP68D

bait
ChAT 

ChAT

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Sadananda et al., 2012)
FBrf0239017-1.FRET.FM
Reference
Dey and Ray, 2018
Description
physical association
Assay
Collection
Source/Stage
larval abdominal lch5 neuron / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ChAT 

ChAT

fluorescence donor

TQ tag

ChAT 

ChAT

fluorescence donor

TQ tag

ChAT 

ChAT

fluorescence donor

TQ tag

Klp68D 

KLP68D

fluorescence acceptor

YFP tag

Klp68D 

KLP68D

fluorescence acceptor

YFP tag

Klp68D 

KLP68D

fluorescence acceptor

YFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

An increase in sFRET was observed at 78 hr AEL. Treatment with Hemicholinium or Bungarotoxin abolished this increment.

(Dey and Ray, 2018)

Source was larval abdominal lch5 neuron of transgenic fly line; fluorescence donor produced from tagged transgenic construct; fluorescence acceptor produced from tagged transgenic construct.

(Dey and Ray, 2018)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)