FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
HDAC1, Mi-2
FlyBase ID
FBig0000108852
Interaction Network
Interactions Browser links
HDAC1 network
Mi-2 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0204378-1.coIP.WB
Reference
Murawska et al., 2008
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mi-2 

Mi-2

bait
HDAC1 

dRPD3

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Murawska et al., 2008)

Positive control

(Murawska et al., 2008)
FBrf0207548-13.coIP.WB
Reference
Kunert et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Kc167
Participants
Corresponds to
Reported as
Role
Note
Mi-2 

dMi-2

bait
HDAC1 

dRPD3

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of Kc167 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Kunert et al., 2009)
FBrf0207548-17.coIP.WB
Reference
Kunert et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Kc167
Participants
Corresponds to
Reported as
Role
Note
HDAC1 

dRPD3

bait
Mi-2 

dMi-2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of Kc167 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Kunert et al., 2009)
FBrf0212010-19.coIP.MS
Reference
Reddy et al., 2010
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mi-2 

Mi-2

bait
HDAC1 

HDAC1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Reddy et al., 2010)
FBrf0212010-31.coIP.WB
Reference
Reddy et al., 2010
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mi-2 

Mi-2

bait
HDAC1 

HDAC1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Reddy et al., 2010)
FBrf0219217-3.coIP.WB
Reference
Fasulo et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mi-2 

dMi-2

bait

FLAG tag

HDAC1 

RPD3

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of Sf9 cell line; bait produced from recombinant baculovirus; prey produced from recombinant baculovirus.

(Fasulo et al., 2012)
FBrf0245834-27.CH.MW
Reference
Mugat et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
OSC
Participants
Corresponds to
Reported as
Role
Note
Mi-2 

Mi-2

neutral component
Mi-2 

Mi-2

neutral component
Mi-2 

Mi-2

neutral component
HDAC1 

Rpd3

neutral component
HDAC1 

Rpd3

neutral component
HDAC1 

Rpd3

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of OSC cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Mugat et al., 2020)

Co-elution of Mi-2, MEP-1, HDAC1, MTA1-like and Caf1-55 in gel filtration captured as all possible binary interactions.

(Mugat et al., 2020)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (5)