FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Cam, Ric
FlyBase ID
FBig0000108696
Interaction Network
Interactions Browser links
Cam network
Ric network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0091220-1.LPD
Reference
Wes et al., 1996
Description
physical association
Assay
Collection
Source/Stage
retina / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cam 

Calmodulin

bait

[125]I label

Ric 

RIC

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Several Ric clones recovered in this phage display screen for Cam binding proteins.

(Wes et al., 1996)

Interaction in vitro; bait obtained from commercial source; prey produced as transgenic fusion protein in bacteriophage (prey from adult retina cDNA expression library).

(Wes et al., 1996)
FBrf0091220-2.FW.TV
Reference
Wes et al., 1996
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ric 

RIC

bait

GST tag or fused to β-gal

Ric 
Ric 
Cam 

Calmodulin

prey

biotin label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Ric 
calmodulin-binding site
sufficient binding region
aa 242-261

coordinates relative to Ric-PA

Ric 
calmodulin-binding site
necessary binding region
aa 255-264

coordinates relative to Ric-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey obtained from commercial source.

(Wes et al., 1996)
FBrf0091220-3.PD.WB
Reference
Wes et al., 1996
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cam 

calmodulin

bait

coupled to agarose

Ric 

RIC

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait obtained from commercial source; prey derived from transgenic adult head extract.

(Wes et al., 1996)
FBrf0091220-4.coIP.WB
Reference
Wes et al., 1996
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cam 

calmodulin

bait
Ric 

RIC

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of wild-type fly line; bait produced from endogenous gene; prey produced from tagged transgenic construct.

(Wes et al., 1996)
FBrf0091220-5.coIP.WB
Reference
Wes et al., 1996
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ric 

RIC

bait

Myc tag

Cam 

calmodulin

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of wild-type fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Wes et al., 1996)
FBrf0105342-2.LPD
Reference
Xu et al., 1998
Description
physical association
Assay
Collection
Source/Stage
retina / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cam 

Calmodulin

bait

[125]I label

Ric 

RIC

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

3 Ric clones recovered in this phage display screen for Cam binding proteins.

(Xu et al., 1998)

Interaction in vitro; bait obtained from commercial source; prey produced as transgenic fusion protein in bacteriophage (prey from adult retina cDNA expression library).

(Xu et al., 1998)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)