Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.
The exd-hth heterodimer and Scr bind cooperatively to DNA; neither exd-hth nor Scr bind the DNA probe tested on their own.
Scr was mixed with a preformed exd-hth heterodimer ("exd-HM") and [32]P labeled DNA containing a fkh egulatory element. DNA-protein complexes observed by EMSA were used to deduce protein-protein interactions.
