FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Klp68D, Klp64D
FlyBase ID
FBig0000108520
Interaction Network
Interactions Browser links
Klp68D network
Klp64D network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0209149-1.coIP.WB
Reference
Doodhi et al., 2009
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Klp68D 

KLP68D

bait
Klp64D 

KLP64D

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Doodhi et al., 2009)
FBrf0209149-15.CH.MW
Reference
Doodhi et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Klp68D 

KLP68D

bait

His tag

Klp68D 
Klp64D 

KLP64D

prey
Klp64D 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Klp64D 
stalk domain
sufficient binding region
aa 425-589
Klp68D 
stalk domain
sufficient binding region
aa 423-584
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Doodhi et al., 2009)
FBrf0209149-18.CH.MW
Reference
Doodhi et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Klp64D 

KLP64D

bait

His tag

Klp64D 
Klp68D 

KLP68D

prey
Klp68D 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Klp64D 
stalk domain
sufficient binding region
aa 425-589
Klp68D 
stalk domain
sufficient binding region
aa 423-584
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Doodhi et al., 2009)
FBrf0209149-3.CS.WB
Reference
Doodhi et al., 2009
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Klp68D 

KLP68D

neutral component
Klp68D 

KLP68D

neutral component
Klp68D 

KLP68D

neutral component
Klp64D 

KLP64D

neutral component
Klp64D 

KLP64D

neutral component
Klp64D 

KLP64D

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Doodhi et al., 2009)
FBrf0209149-7.CH.WB
Reference
Doodhi et al., 2009
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Klp68D 

KLP68D

bait

His tag

Klp64D 

KLP64D

prey
Klp64D 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Klp64D 
stalk domain
sufficient binding region
aa 425-589
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type adult head extract.

(Doodhi et al., 2009)
FBrf0218568-4.PD.WB
Reference
Sadananda et al., 2012
Description
physical association
Assay
Collection
Source/Stage
head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Klp64D 

KLP64D

bait

His tag

Klp64D 
Klp68D 

KLP68D

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Klp64D 
tail fragment
sufficient binding region
aa 590-677
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from adult heads of transgenic fly line.

(Sadananda et al., 2012)
FBrf0219545-1.CH.WB
Reference
Doodhi et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Klp64D 

Klp64D

bait

His tag

Klp64D 
Klp64D 
Klp68D 

Klp68D

prey
Klp68D 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Klp64D 
interaction domain
sufficient binding region
aa 425-528aa 507-589
Klp64D 
interaction domain
necessary binding region
aa 507-589
Klp68D 
interaction domain
sufficient binding region
aa 423-584
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

(Doodhi et al., 2012)

Klp64D and Klp68D copurify with each other when one is tagged and both are expressed in a bacterial system.

(Doodhi et al., 2012)
FBrf0219545-2.CH.WB
Reference
Doodhi et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Klp68D 

Klp68D

bait

His tag

Klp68D 
Klp68D 
Klp64D 

Klp64D

prey
Klp64D 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Klp64D 
interaction domain
sufficient binding region
aa 425-589
Klp68D 
interaction domain
sufficient binding region
aa 423-584aa 521-784
Klp68D 
interaction domain
necessary binding region
aa 502-584aa 423-501
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

(Doodhi et al., 2012)

Klp64D and Klp68D copurify with each other when one is tagged and both are expressed in a bacterial system.

(Doodhi et al., 2012)
FBrf0219545-3.CH.MW
Reference
Doodhi et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Klp64D 

Klp64D

neutral component

His tag

Klp64D 

Klp64D

neutral component

His tag

Klp64D 
Klp64D 

Klp64D

neutral component

His tag

Klp64D 
Klp68D 

Klp68D

neutral component

His tag

Klp68D 
Klp68D 

Klp68D

neutral component

His tag

Klp68D 

Klp68D

neutral component

His tag

Klp68D 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Klp64D 
interaction domain
sufficient binding region
aa 425-589
Klp68D 
interaction domain
sufficient binding region
aa 423-584
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

(Doodhi et al., 2012)
FBrf0219545-4.CD
Reference
Doodhi et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Klp64D 

Klp64D

neutral component

His tag

Klp64D 

Klp64D

neutral component

His tag

Klp64D 

Klp64D

neutral component

His tag

Klp68D 

Klp68D

neutral component

His tag

Klp68D 

Klp68D

neutral component

His tag

Klp68D 

Klp68D

neutral component

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

(Doodhi et al., 2012)
FBrf0234587-1.coIP.WB
Reference
Girotra et al., 2017
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Klp64D 

KLP64D

bait

His tag

Klp68D 

KLP68D

prey

YFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

(Girotra et al., 2017)
FBrf0243259-1.FRET
Reference
Ahmed et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Klp64D 

KLP64D

fluorescence donor

CFP tag

Klp64D 

KLP64D

fluorescence donor

CFP tag

Klp64D 
Klp64D 

KLP64D

fluorescence donor

CFP tag

Klp64D 
Klp68D 

KLP68D

fluorescence acceptor

YFP tag

Klp68D 
Klp68D 

KLP68D

fluorescence acceptor

YFP tag

Klp68D 

KLP68D

fluorescence acceptor

YFP tag

Klp68D 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Klp64D 
stalk domain
sufficient binding region
aa 425-589
Klp68D 
stalk domain
sufficient binding region
aa 423-584
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; fluorescence donor produced as a recombinant fusion protein in bacterial system; fluorescence acceptor produced as a recombinant fusion protein in bacterial system.

(Ahmed et al., 2019)
FBrf0243259-2.coIP.WB
Reference
Ahmed et al., 2019
Description
physical association
Assay
Collection
Source/Stage
organism / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Klp68D 

KLP68D

bait
Klp64D 

KLP64D

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larvae of wild-type or mutant fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Ahmed et al., 2019)
FBrf0244469-1.FRET.FM
Reference
Ahmed et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Klp64D 

KLP64D

fluorescence donor

mTurquoise tag

Klp64D 
Klp64D 
Klp64D 

KLP64D

fluorescence donor

mTurquoise tag

Klp64D 
Klp64D 
Klp64D 

KLP64D

fluorescence donor

mTurquoise tag

Klp68D 
Klp68D 

KLP68D

fluorescence acceptor

YFP tag

Klp68D 

KLP68D

fluorescence acceptor

YFP tag

Klp68D 
Klp68D 

KLP68D

fluorescence acceptor

YFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Klp64D 
stalk domain
sufficient binding region
aa 425-589
Klp68D 
stalk domain
sufficient binding region
aa 423-584
Klp64D 
stalk domain residue 551
mutation increasing interaction
aa 551

E551K

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; fluorescence donor produced as a recombinant fusion protein in bacterial system; fluorescence acceptor produced as a recombinant fusion protein in bacterial system.

(Ahmed et al., 2020)

The Klp64D-Klp68D heterodimer is structurally dynamic. FRET studies using fluorophores at either the N- or C-termini suggest that both ends are equally dynamic. Binding of Kap3 increases the FRET efficiency of the C-terminal end of the Klp64D-Klp68D heterodimer.

(Ahmed et al., 2020)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (6)