FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Rm62, Su(var)3-9
FlyBase ID
FBig0000108335
Interaction Network
Interactions Browser links
Rm62 network
Su(var)3-9 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0213941-1.CH.MS
Reference
Boeke et al., 2011
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Su(var)3-9 

Su(var)3-9

bait

GST tag

Su(var)3-9 
Rm62 

Rm62

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Su(var)3-9 
interaction domain
sufficient binding region
aa 1-152
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.

(Boeke et al., 2011)
FBrf0213941-2.coIP.WB
Reference
Boeke et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Rm62 

Rm62

bait
Su(var)3-9 

Su(var)3-9

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear cell extract of S2 cell line; bait derived from endogenous gene; prey produced from transfected construct.

(Boeke et al., 2011)
FBrf0213941-3.PD.A
Reference
Boeke et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rm62 

Rm62

bait

GST tag

Su(var)3-9 

Su(var)3-9

prey

[35]S label

Su(var)3-9 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Su(var)3-9 
interaction domain
necessary binding region
aa 1-152
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Boeke et al., 2011)
FBrf0213941-5.PD.A
Reference
Boeke et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Su(var)3-9 

Su(var)3-9

bait

GST tag

Su(var)3-9 
Rm62 

Rm62

prey

[35]S label

Rm62 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Su(var)3-9 
interaction domain
sufficient binding region
aa 1-152
Rm62 
interaction domain
sufficient binding region
aa 1-141
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Boeke et al., 2011)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)